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Mouse apolipoprotein AI. cDNA-derived primary structure, gene organisation and complete nucleotide sequence
Biological Chemistry Hoppe-Seyler
|April 1, 1992
Summary
Researchers isolated the mouse apolipoprotein AI (Apo AI) gene, crucial for cholesterol transport. This cDNA clone and gene sequence enable functional studies of Apo AI in mice.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Apolipoprotein AI (Apo AI) is central to cholesterol homeostasis and reversed cholesterol transport.
- Apo AI activates lecithin-cholesterol acyltransferase and acts as a ligand for HDL receptors.
Purpose of the Study:
- To isolate and characterize the full-length mouse apolipoprotein AI (Apo AI) gene.
- To facilitate functional analysis of Apo AI through transgenic mouse models.
Main Methods:
- Isolation of a full-length mouse liver cDNA clone encoding Apo AI.
- Nucleotide sequencing of the cDNA and comparison with rat and human sequences.
- Isolation and analysis of the mouse Apo AI gene organization, including exons and introns.
Main Results:
- A mouse Apo AI cDNA clone (892 bp) was obtained, coding for 264 amino acids.
- The mouse Apo AI sequence showed 70.7% homology to rat and 66% to human sequences.
- The mouse Apo AI gene structure (four exons, three introns) is similar to the human gene, with significant sequence divergence in introns.
Conclusions:
- The isolated mouse Apo AI cDNA and gene provide essential tools for studying Apo AI function in cholesterol metabolism.
- Comparative analysis reveals conserved and divergent features between mouse, rat, and human Apo AI genes, particularly in intronic regions.