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Updated: Aug 17, 2026
A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms
Published on: September 16, 2022
A high-throughput colorimetric and fluorometric microassay for the evaluation of nitroimidazole derivatives
Ma Elena Campos Aldrete1, Héctor Salgado-Zamora, Julieta Luna
1Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas-IPN, Prol. Carpio y Plan de Ayala, S/N CP 11340 México, D.F., Mexico. camesol22@hotmail.com
Abstract:
Several nitroimidazole derivatives were synthesized and tested as possible trichomonicidal agents. A fast, simple, practical and reliable in vitro colorimetric method was applied to the screening of the nitroimidazole derivatives anti-trichomonas activity. The colorimetric technique was based on the use of Alamar blue as a redox-indicator. The test was carried out both qualitatively (minimal inhibitory concentration determined by naked eye observation) and quantitatively (fluorometric determination of 50% and 90% inhibitory concentrations), the latter took advantage of the dye fluorometric properties. The performance of the method was excellent affording an exactitude 97.86% and a reproducibility of 95% and no interference of the trichomonas-culture medium was observed during the test. Some of the nitroimidazole compounds tested showed a fair trichomonicidal activity, however none of them was as active as the model compound, metronidazole.
