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Humoral autoimmunity in mice overexpressing B cell surface CD19: vital role for MHC class II

William Stohl1, Dong Xu, Kyoung Soo Kim

  • 1Division of Rheumatology and Immunology, Department of Medicine, University of Southern California Keck School of Medicine, 2001 Zonal Avenue HMR 711, Los Angeles, CA 90033, USA. stohl@usc.edu

Clinical Immunology (Orlando, Fla.)
|June 21, 2005
PubMed

B6 mice bearing a transgene (Tg) for human CD19 (hCD19) harbor increased numbers of splenic Ig-secreting cells (IgSC), increased serum levels of total Ig and autoantibodies, but decreased numbers of splenic B cells. To assess the influence of MHC class II (MHCII) to this phenotype, MHCII-deficient CD19-Tg mice were generated. Compared to MHCII-sufficient CD19-Tg mice, splenic IgSC numbers and serum Ig and autoantibody levels were markedly diminished, and the decrease in splenic B cell numbers was aggravated. Remarkably, genetic reconstitution of these MHCII-deficient mice with a human DQ8 Tg resulted in a hierarchical restorative pattern. Restoration of splenic B cell numbers was complete; restoration of numbers of splenic IgSC and of serum Ig levels was partial; and restoration of circulating autoantibody levels was virtually non-existent. Thus, MHCII expression has a profound effect on B cells which can be uncoupled from global Ig and autoantibody production in the hCD19-Tg model. This raises the possibility that MHCII affects B cells in a manner that, at least in part, is independent of helper T cell function.

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