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An Improved Method for the Preparation of Type I Collagen From Skin
Published on: January 21, 2014
Isolation and characterization of collagen from brown backed toadfish (Lagocephalus gloveri) skin
L S Senaratne1, Pyo-Jam Park, Se-Kwon Kim
1Department of Chemistry, Pukyong National University, 599-1, Daeyeon-3-dong, Nam-Gu, Busan 608-737, Republic of Korea.
Abstract:
Collagen was isolated and characterized from the skin of brown backed toadfish (Lagocephalus gloveri), processing wastes. The total collagen yield extracted was 54.3% on the basis of lyophilized dry weight compared to other vertebrates. According to the electrophoretic pattern, the skin collagen of the fish consisted of heterotrimer alpha1alpha2alpha3. Moreover, the denaturation temperature was 28 degrees C, which was about 9 degrees C lower than that of collagen from porcine skin. Therefore, there is a possibility to use brown backed toadfish skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.

