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Updated: Aug 17, 2026

Lipid Exchange Assay in Living Cells
Published on: March 21, 2025
Interleukin-2alpha receptor in membrane lipid rafts
1Research Institute of General Surgery, Nanjing General Hospital of Nanjing Command, Nanjing 210002, People's Republic of China. liqiurong@yahoo.com
Abstract:
Acute allograft rejection is driven by production of cytokines such as interleukin(IL)-2 that activate and expand alloreactive T cells by ligating high-affinity IL-2alpha receptors. Lipid rafts on the membranes of T cells may be the functional microdomains for IL-2alpha receptor. Flow cytometric analysis was performed for expression of CD25 (IL-2alpha receptor) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Ralpha in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Cells were stimulated with IL-2 and expression of CD25 (IL-2alpha receptor) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Ralpha was localized in lipid rafts. Lipid rafts are the functional microdomains for IL-2alpha receptors.
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