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Stab-Wound Mouse Model for Studying Hemorrhage and Inflammation in Traumatic Brain Injury
Published on: February 21, 2025
Immune pre-activation exacerbates hemorrhagic brain injury in immature mouse brain
Mengzhou Xue1, Marc R Del Bigio
1Department of Pathology, University of Manitoba and Manitoba Institute of Child Health, D212-770 Bannatyne Ave, Winnipeg, MB, Canada, R3E 0W3.
Abstract:
Premature infants with placental infection and adult stroke patients with fever have worse outcomes following intracerebral hemorrhage (ICH). We hypothesized that immune pre-activation would aggravate brain injury in mouse brain following ICH. The immune system of 2-day, 10-day and 7-week young adult CD1 mice was stimulated by intraperitoneal injection of concanavalin A (ConA), lipopolysaccharide (LPS) or polyinosinic-polycytidilic acid (PolyI:C) 12 h prior to intracerebral injection of blood. Two days later, brain damage and inflammation were worse in 2-day mice that had received LPS. The other agents had less consistent effects in 2-day mice. Brain damage in young adults was aggravated less after immune stimulation. These data suggest that immune pre-activation modifies hemorrhagic brain injury in immature mouse brain.
