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Immunoblot analysis of specific antigen bands predictable for Dirofilaria immitis infection in cats

Mineo Hayasaki1, Naoko Mori, Haruka Hongo

  • 1Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. hayasaki@yamaguchi-u.ac.jp

Veterinary Parasitology
|June 21, 2005
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.