Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Compound transfer efficiency from polystyrene surfaces: application to microarrayed compound screening.

Xueheng Cheng1, Bing Yan, Lan Gao

  • 1Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064, USA. xueheng.cheng@abbott.com

Journal of Biomolecular Screening
|June 21, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alleviation of Pb<sup>2+</sup> pollution-induced oxidative stress and toxicity in microglial cells and zebrafish larvae by chicoric acid.

Ecotoxicology and environmental safety·2019
Same author

MIL-61 and Eu<sup>3+</sup>@MIL-61 as Signal Transducers To Construct an Intelligent Boolean Logical Library Based on Visualized Luminescent Metal-Organic Frameworks.

ACS applied materials & interfaces·2019
Same author

Stem Cell-Derived Exosomes Prevent Aging-Induced Cardiac Dysfunction through a Novel Exosome/lncRNA MALAT1/NF-<i>κ</i>B/TNF-<i>α</i> Signaling Pathway.

Oxidative medicine and cellular longevity·2019
Same author

Human umbilical cord mesenchymal stem cell derived exosomes encapsulated in functional peptide hydrogels promote cardiac repair.

Biomaterials science·2019
Same author

Carbohydrate metabolism genes dominant in a subtropical marine mangrove ecosystem revealed by metagenomics analysis.

Journal of microbiology (Seoul, Korea)·2019
Same author

The Diagnostic Value of 3D Power Doppler Ultrasound Combined With VOCAL in the Vascular Distribution of Breast Masses.

Academic radiology·2019

Compound transfer in microarrayed compound screening (microARCS) is primarily governed by dissolution kinetics, not equilibrium solubility. Lower ClogP compounds transfer faster, ensuring sufficient amounts for screening assays.

Area of Science:

  • Drug discovery and development
  • Chemical biology
  • Assay development

Background:

  • Microarrayed compound screening (microARCS) utilizes high-density compound arrays on ChemCards for drug screening.
  • Efficient compound transfer from the ChemCard to assay reagents (e.g., agarose gels) is crucial for assay success.

Purpose of the Study:

  • To characterize the compound transfer process in microARCS.
  • To identify key factors influencing compound transfer efficiency from ChemCards to agarose gels.

Main Methods:

  • Investigated compound transfer using 59 diverse compounds on ChemCards.
  • Quantified remaining compounds via liquid chromatography/mass spectrometry after incubation with agarose gels.
  • Correlated transfer efficiency with compound properties like solubility and ClogP.

Related Experiment Videos

Main Results:

  • Compound transfer correlated well with transfer kinetics to PBS buffer but moderately with equilibrium solubility.
  • Dissolution kinetics, rather than solubility or diffusion, was the primary determinant of transfer efficiency.
  • Lower ClogP compounds exhibited faster transfer rates.
  • Compound transfer was sufficient for screening purposes (>20% in 10 min for most compounds).

Conclusions:

  • Compound transfer in microARCS is mainly controlled by dissolution rate.
  • ClogP is a significant factor affecting transfer kinetics.
  • The microARCS method provides adequate compound amounts for effective screening.