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Characteristics and subcellular localization of pristanoyl-CoA synthetase in rat liver
R J Wanders1, S Denis, C W van Roermund
1Department of Clinical Biochemistry (FO-226), University of Amsterdam, Netherlands.
Biochimica Et Biophysica Acta
|May 8, 1992
Abstract:
We have investigated the activation of pristanic acid to its CoA-ester in rat liver. The results show that peroxisomes, mitochondria as well as microsomes contain pristanoyl-CoA synthetase activity. On the basis of competition experiments and immunoprecipitation studies using antibodies raised against rat liver microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3) we conclude that pristanic acid is activated by the same enzyme which activates long-chain fatty acids, i.e., long-chain fatty acyl-CoA synthetase.