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Updated: Aug 17, 2026

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
Published on: August 4, 2017
Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part III. Hydrogels as carriers for immobilization of
J Michálek1, M Prádný, A Artyukhov
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic. jiri@imc.cas.cz
Abstract:
Four series of macroporous hydrogels based on crosslinked copolymers of 2-hydroxyethyl methacrylate (HEMA)-sodium methacrylate (MANa), copolymer HEMA-[2-(methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl), terpolymer HEMA-MANa-MOETACl and on a polyelectrolyte complex were used as carriers for immobilization of proteins, chicken egg white albumin and avidin. The adsorption capacity of the hydrogels for the two proteins, kinetics and pH dependence of albumin adsorption and desorption were studied. The morphology of the hydrogels with and without immobilized albumin was studied by low-vacuum scanning electron microscopy.
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