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Related Experiment Videos

GI flexible endoscope disinfection: "in use" test comparative study.

Josep M Bordas1, M Angeles Marcos-Maeso, M Jesus Perez

  • 1GI Endoscopy, Motility and Functional Tests, Institut de Malalties Digestives, Institut Malalties Digestives, Hospital Clinic, Facultat de Medicina Universitat de Barcelona, Barcelona, Spain. jmbordas@clinic.ub.es

Hepato-Gastroenterology
|June 22, 2005
PubMed
Summary

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This study evaluates disinfectants for high-level disinfection, finding several effective alternatives to alkaline-glutaraldehyde. A sensitive microbiologic method aids in assessing disinfectant efficacy and establishing regulations.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Healthcare Disinfection

Background:

  • Confusion exists regarding the effectiveness of disinfectants for high-level disinfection.
  • Assessing disinfectant efficacy requires a reliable microbiologic method.
  • The "in use" method is suitable for comparing disinfectants.

Purpose of the Study:

  • To compare the efficacy of various disinfectants and disinfection regimens.
  • To evaluate automated disinfection systems against standard methods.
  • To validate a microbiologic method for assessing high-level disinfection.

Main Methods:

  • Disinfectants including alkaline-glutaraldehyde, glutaraldehydephenol-phenate, peracetic acid, and ortho-phthalaldehyde were tested.
  • Manual and automated disinfection methods were employed.

Related Experiment Videos

  • Effluent microbial growth was assessed using thioglycolate broth, blood agar, and MacConkey agar.

    • Main Results:

    • Several disinfectants demonstrated efficacy comparable to or exceeding 2% alkaline-glutaraldehyde.
    • Optimal bacterial reduction (>3 log10) was achieved with specific concentrations and contact times of glutaraldehydephenol-phenate, peracetic acid, hydrogen peroxide, alkaline-glutaraldehyde, and ortho-phthalaldehyde.
    • Automated systems were included in the comparison.

    Conclusions:

    • A sensitive microbiologic method can effectively control disinfection processes.
    • This method clarifies the efficacy limits of common disinfection practices.
    • The study highlights the need for microbiologic regulations in high-level disinfection assessment.