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Updated: Jul 2, 2026

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
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[Studies on immobilized cellobiase].

Xue-Liang Shen1, Li-Ming Xia

  • 1Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 22, 2005
PubMed
Summary
This summary is machine-generated.

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Immobilizing Aspergillus niger spores in calcium alginate enhances cellobiase activity for efficient cellulosic biomass conversion. This method improves glucose yields from agricultural residues, supporting sustainable biofuel and chemical production.

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Renewable Energy

Background:

  • Cellulosic biomass is a key renewable resource for biofuels and chemicals.
  • Cellulase enzyme complex, including cellobiase, is crucial for cellulose hydrolysis.
  • Low cellobiase activity in Trichoderma reesei cellulase limits glucose yield.

Purpose of the Study:

  • To develop an efficient method for cellobiase immobilization.
  • To enhance the saccharification yield of cellulosic materials.
  • To improve the conversion of biomass into valuable products.

Main Methods:

  • Immobilization of Aspergillus niger spores (rich in cellobiase) in calcium alginate gels.
  • Characterization of immobilized cellobiase stability, pH, and kinetic properties.

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Last Updated: Jul 2, 2026

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  • Application in repeated batch and continuous hydrolysis of cellobiose and corncob residue.

    • Main Results:

    • Immobilized cellobiase exhibited high stability (38-day half-life at 50°C) and thermal stability (stable below 70°C).
    • Optimal activity at pH 4.8, with Km of 6.01 mmol/L and Vmax of 7.06 mmol/min·L.
    • Achieved >97% hydrolysis yield in 10 batches and 98% in continuous flow, significantly improving corncob residue hydrolysis yield from 69.5% to 84.2%.

    Conclusions:

    • Spore-based cellobiase immobilization is an effective strategy to enhance biomass saccharification.
    • The immobilized enzyme improves glucose yield and reduces fermentation inhibition.
    • This approach offers a sustainable pathway for converting lignocellulosic biomass into valuable chemicals and biofuels.