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Superoxide dismutase and glutathione peroxidase enzyme activities in larynx carcinoma
Abdullah Kalayci1, Adnan Ozturk, Kayhan Ozturk
1Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Selcuk University, Konya, Turkey. abdullahkalayci@hotmail.com
Conclusions:
It can be concluded that these changes are related to damage at the DNA level or to the inhibitory effects of tumor promoters. Increases in GSH-Px activities may be related to the independence of this enzyme from the suppressive effects of tumor promoters. This study and others in the literature show that it is not possible to generalize the activities of SOD and GSH-Px in cancer.
Objective:
There has been growing interest in the role of free radicals as a cause of cancer. It has been suggested that an increase in activated forms of oxygen in cells due to overproduction and/or the inability to destroy them may lead to severe damage of cell structures. As a result of these changes, some chromosomal aberrations and carcinogenesis may develop. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) are two important antioxidant enzymes involved in enzymatic antioxidant defense mechanisms. To our knowledge there have been no previous studies in the literature investigating the activities of SOD and GSH-Px in laryngeal cancer.
Material And Methods:
The study subjects comprised 10 male patients (age range 43-76 years) with laryngeal carcinoma and 10 healthy controls (4 males, 6 females; age range 40-69 years) with intraoral hyperplastic fibrous tissue. Homogenate SOD and GSH-Px activities were measured using commercially available kits.
Results:
GSH-Px levels were significantly increased in the cancerous tissues compared with cancer-free adjacent tissues and fibrous hyperplasia tissues (p < 0.05), whereas there was no significant difference between SOD activities (p > 0.05). There was also no significant difference between GSH-Px activity in cancer-free adjacent tissues and fibrous hyperplasia tissues (p > 0.05).
Insights
Laryngeal cancer shows increased glutathione peroxidase (GSH-Px) activity, but superoxide dismutase (SOD) activity remains unchanged. These findings suggest DNA damage or tumor promoter effects, highlighting that SOD and GSH-Px activities cannot be generalized across all cancers.
Area of Science:
- Biochemistry
- Oncology
- Free Radical Biology
Background:
- Free radicals and reactive oxygen species are implicated in carcinogenesis.
- Antioxidant enzymes like superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) are crucial for cellular defense.
- Limited research exists on SOD and GSH-Px activity in laryngeal cancer.
Purpose of the Study:
- To investigate the activity of SOD and GSH-Px in laryngeal cancer tissues.
- To explore the potential role of these enzymes in the context of cancer development and tumor promotion.
Main Methods:
- Enzyme activity assays for SOD and GSH-Px were performed using commercial kits.
- Study included 10 male laryngeal carcinoma patients and 10 healthy controls.
- Comparison was made between cancerous tissues, adjacent non-cancerous tissues, and control tissues.
Main Results:
- Glutathione peroxidase (GSH-Px) activity was significantly elevated in laryngeal cancer tissues compared to controls (p < 0.05).
- Superoxide dismutase (SOD) activity showed no significant difference between cancer and control groups (p > 0.05).
- GSH-Px activity did not differ significantly between adjacent non-cancerous tissues and control tissues.
Conclusions:
- Elevated GSH-Px activity in laryngeal cancer may be linked to DNA damage or tumor promoter effects.
- GSH-Px may maintain activity independently of tumor promoter inhibition.
- SOD and GSH-Px activities are not universally generalizable in cancer research.