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Updated: Jul 16, 2026
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Principles and applications of multidimensional protein identification technology.
Andrew C Paoletti1, Boris Zybailov, Michael P Washburn
1Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110, USA. acp@stowers-institute.org
Expert Review of Proteomics
|June 22, 2005
Summary
Multidimensional protein identification technology (MudPIT) uses advanced chromatography and mass spectrometry for comprehensive proteomic analysis. This powerful technique enables researchers to analyze complex biological samples like whole proteomes.
Area of Science:
- Proteomics
- Analytical Chemistry
- Biochemistry
Background:
- Multidimensional chromatography coupled with tandem mass spectrometry is an emerging technique for analyzing proteomes.
- This technology is being rapidly adopted by researchers for proteomic analysis.
Purpose of the Study:
- To discuss a specific proteomic approach known as multidimensional protein identification technology (MudPIT).
Main Methods:
- MudPIT utilizes a biphasic microcapillary column packed with reversed-phase and strong cation-exchange materials.
- A complex peptide mixture is loaded onto the column, which is then connected in-line with quaternary high-performance liquid chromatography and a tandem mass spectrometer.
Main Results:
- MudPIT demonstrates the capability to analyze highly complex proteomic mixtures.
- This includes the analysis of whole proteomes, organelles, and protein complexes.
Conclusions:
- MudPIT is a powerful and versatile technique for in-depth proteomic analysis.
- Its application extends to the characterization of complex biological systems.
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