Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Biological samples have distinct optical characteristics in the short-wave infrared (SWIR) compared to the visible (VIS) and near-infrared (NIR) wavelength ranges. However, recording pure SWIR absorption spectra is challenging and rarely conducted for biological molecules. This article presents a method to characterize the VIS-SWIR absorption spectra of biological...

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample...

Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids

Excitonic Hamiltonians for Calculating Optical Absorption Spectra and Optoelectronic Properties of Molecular Aggregates and Solids

Here, we present a protocol for parametrizing a tight-binding excitonic Hamiltonian for calculating optical absorption spectra and optoelectronic properties of molecular materials from first-principles quantum chemical...

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Here, we present a protocol for typical experiments of soft X-ray absorption spectroscopy (sXAS) and resonant inelastic X-ray scattering (RIXS) with applications in battery material...

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.

Emission Spectra

Emission Spectra

When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Femtosecond Soft X-ray Absorption Spectroscopy Identifies Metal-Centered S<sub>1</sub> Excited State of Cyanocobalamin.

Journal of the American Chemical Society·2026

Same author

Ultrafast Relaxation of MLCT Excited States in Manganese Tricarbonyl Complexes: Insights from Polarization-Resolved Femtosecond X-ray Absorption Spectroscopy at the Mn and Br K-Edges.

Journal of the American Chemical Society·2026

Same author

Probing anion recognition in a cobalt(II) de novo designed metalloprotein.

Journal of inorganic biochemistry·2026

Same author

Ultrafast solvent-modulated roaming mechanism in bromoform revealed by femtosecond X-ray solution scattering.

Nature communications·2026

Same author

Blank Spectrum Correction as a Robust Solution to Artifacts in Quantitative X-ray Fluorescence Mapping.

bioRxiv : the preprint server for biology·2026

Same author

Shining light on the mechanism of photochemical alkene formation in vitamin B<sub>12</sub>.

Chemical science·2026

Same journal

Launching a new era for Short Communications in Journal of Synchrotron Radiation.

Journal of synchrotron radiation·2026

Same journal

Sagittal collimating diaboloid: a new grazing-incidence mirror surface for higher-throughput resonant inelastic X-ray scattering spectrometers.

Journal of synchrotron radiation·2026

Same journal

Synchrotron X-ray tomography and spectroscopy in numismatics: disclosing counterfeit practices in medieval silver coins.

Journal of synchrotron radiation·2026

Same journal

The Big Data Science Center at the Shanghai Synchrotron Radiation Facility: the architecture of the superfacility.

Journal of synchrotron radiation·2026

Same journal

A robotic and high-throughput X-ray micro-computed tomography workflow.

Journal of synchrotron radiation·2026

Same journal

Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.

Journal of synchrotron radiation·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 20, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

A method for normalization of X-ray absorption spectra.

Tsu-Chien Weng¹, Geoffrey S Waldo, James E Penner-Hahn

¹Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109-1055, USA.

Journal of Synchrotron Radiation

|June 22, 2005

Summary

This summary is machine-generated.

A new method, MBACK, accurately normalizes X-ray absorption data. This approach improves reliability and reduces errors compared to conventional techniques, enabling precise quantitative analysis of near-edge features.

More Related Videos

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Published on: May 27, 2022

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

Related Experiment Videos

Last Updated: Jan 20, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

Published on: January 10, 2025

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Published on: May 27, 2022

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

Area of Science:

Materials Science
Spectroscopy
Analytical Chemistry

Background:

Quantitative analysis of X-ray absorption (XAS) data relies on accurate normalization.
Conventional normalization methods can be sensitive to background function shapes, introducing errors.

Purpose of the Study:

To introduce and validate a new method, MBACK, for normalizing X-ray absorption data.
To demonstrate MBACK's improved accuracy and reliability over conventional techniques.

Main Methods:

Implementation of the MBACK program for data normalization.
Comparison of MBACK with conventional normalization methods.
Analysis of XAS data collected during an equilibrium titration to assess sensitivity.

Main Results:

MBACK normalization is insensitive to background function shape.
MBACK allows accurate comparison of XAS data from different detection modes (transmission, fluorescence).
MBACK exhibits enhanced reliability, consistency, and reduced errors compared to conventional methods.

Conclusions:

MBACK provides a robust and accurate method for X-ray absorption data normalization.
The improved normalization facilitates more reliable quantitative analysis of XAS near-edge features.
MBACK enhances the comparability of XAS data across different experimental setups.