Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Oxidative refolding of proteins].

Yu-Ying Zhang1, Kai-Yu Yang

  • 1Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 23, 2005
PubMed
Summary

Protein oxidative refolding often involves multiple pathways, guided by native interactions. Strategies to favor productive intermediates enhance renaturation yields for recombinant proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of neoadjuvant chemoradiotherapy resistance-associated proteins in locally advanced rectal cancer: A pilot study.

Oncology letters·2026
Same author

Chemosensory System Decoding: Transcriptome-Wide Identification and Expression Profiling of Olfactory Genes in <i>Lytta sifanica</i>.

Ecology and evolution·2025
Same author

Retraction Note: MiR-203a-3p regulates the biological behaviors of ovarian cancer cells through mediating the Akt/GSK-3β/Snail signaling pathway by targeting ATM.

Journal of ovarian research·2025
Same author

Synthesis of tetrahydropyrrolo[1,2-<i>a</i>]quinolinone analogues <i>via</i> CoCl<sub>2</sub>/AgSbF<sub>6</sub>-catalyzed cyclization of unactivated <i>N</i>,<i>O</i>-acetals with olefins.

Organic & biomolecular chemistry·2025
Same author

Sleep quality, APOE ε4, and Alzheimer's disease: associations from two prospective cohort studies and mechanisms by plasma proteomic analysis.

BMC medicine·2025
Same author

Low-Electromotive Force-Driven Sodium Compensation for Optimizing Na Deposition in Rechargeable Sodium Batteries.

Journal of the American Chemical Society·2025

Area of Science:

  • Protein folding and biophysics
  • Biochemistry and molecular biology

Context:

  • Oxidative refolding is crucial for producing functional disulfide-containing proteins.
  • Understanding protein folding mechanisms is key to overcoming renaturation challenges.
  • Recombinant protein yield is often limited by aggregation and mispaired disulfide bonds.

Purpose:

  • To elucidate the detailed mechanism of protein oxidative refolding.
  • To identify factors influencing folding pathways and disulfide bond formation.
  • To provide strategies for enhancing the renaturation yield of recombinant proteins.

Summary:

  • Protein oxidative refolding proceeds via multiple pathways, influenced by native interactions.
  • Disulfide bond formation is interdependent with native structure recovery, especially in later stages.
  • Productive intermediates with native-like structures are essential for correct refolding and high yields.

Impact:

  • Provides insights into optimizing recombinant protein production in biotechnology.
  • Offers strategies to destabilize unproductive intermediates and promote native disulfide bond formation.
  • Highlights the importance of conformational flexibility in productive folding intermediates.

Related Experiment Videos