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Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
Published on: November 18, 2013
[Isolation and identification of reprogramming genes related to the development of the rabbit reconstructed embryos]
Wei-Dong Yu1, Wen-Yong Li, Yu-Ge Wang
1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Graduated School of Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
By the method of single preimplantation embryos differential display polymerase chain reaction (SPEDDRT-PCR), 25 reprogramming cDNA fragments were obtained from single 2-cell, 8-cell embryos and blastula. After cloning and sequencing, five of them were identified by reverse-Northern and characterized with stage-specific expression during reconstructed embryo development. This results will help to isolate full length reprogramming genes and study their function during embryonic development.
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