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Related Experiment Videos

[Study of the expression membrane protein LASS2].

Xing-Feng Cai1, Zhen Tao, Yu Cao

  • 1Shanghai Research Center of Biotechnology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 23, 2005
PubMed
Summary

Researchers successfully expressed the full-length human LASS2 protein using eukaryotic and baculovirus systems. This advancement aids in studying the LASS2 membrane protein

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Area of Science:

  • Molecular Biology
  • Protein Expression
  • Biochemistry

Background:

  • LASS2 (longevity assurance homologue 2) is a human membrane protein with significant physiological roles.
  • Efficient expression systems are crucial for studying LASS2 protein functions.

Purpose of the Study:

  • To express and purify the full-length human LASS2 protein.
  • To develop tools for LASS2 protein research.

Main Methods:

  • Prokaryotic expression for LASS2 carboxyl terminal fragment and antibody production.
  • Eukaryotic in vitro translation and Baculovirus (Bac-to-Bac) system for full-length LASS2 expression.
  • SDS-PAGE, Western blot, and metal affinity chromatography for analysis and purification.

Main Results:

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  • The LASS2 carboxyl terminal fragment was expressed in prokaryotes, enabling polyclonal antibody production.
  • Full-length LASS2 protein was successfully expressed in eukaryotic in vitro and Baculovirus systems.
  • Purified recombinant LASS2 protein showed a molecular weight of approximately 28 kD with >90% purity.

Conclusions:

  • The Bac-to-Bac system provides an effective method for expressing and purifying full-length human LASS2 protein.
  • The developed recombinant LASS2 protein and antibody are valuable reagents for further biological studies.