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[Effects of bkdAB interruption on avermectin biosynthesis].

Hao-Jun Zhu1, Yun-Xiang Liang, Jun-Chu Zhou

  • 1Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 23, 2005
PubMed
Summary

Streptomyces avermitilis Bjbm0006 gene cluster bkdAB interruption resulted in loss of avermectin B

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Context:

  • Streptomyces avermitilis Bjbm0006 is a known producer of four avermectin B components.
  • Avermectin B biosynthesis involves complex metabolic pathways.
  • Understanding gene function is crucial for metabolic engineering of antibiotic production.

Purpose:

  • To investigate the role of the bkdAB gene cluster in avermectin B biosynthesis.
  • To determine the impact of bkdAB gene disruption on avermectin B component production.
  • To identify novel compounds produced by genetically modified strains.

Summary:

  • A replacement plasmid pHJ5821 was constructed to interrupt the bkdAB gene cluster in S. avermitilis Bjbm0006.
  • The resulting recombinant strain Bjbm5821 lost the ability to produce 'b' avermectin B components (e.g., B1b, B2b).

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  • HPLC analysis confirmed the absence of 'b' components and the presence of a novel compound, suggesting bkdAB's role in avermectin synthesis.

    • Impact:

    • The bkdAB gene cluster, encoding branched-chain alpha-keto acid dehydrogenase, plays a significant role in avermectin B biosynthesis.
    • This finding provides insights into the specific functions of genes within the avermectin pathway.
    • Results can guide future metabolic engineering strategies for optimizing avermectin production.