Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ouabain-induced cell signaling.

Steven Harwood1, Muhammad M Yaqoob

  • 1Centre for Experimental Medicine, Nephrology, and Critical Care, William Harvey Research Institute, John Vane Science Centre, Charterhouse Square, London EC1M 6BQ, United Kingdom. s.m.harwood@qmul.ac.uk

Frontiers in Bioscience : a Journal and Virtual Library
|June 23, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: IkB Kinase Inhibitor Attenuates Sepsis-Induced Cardiac Dysfunction in CKD.

Journal of the American Society of Nephrology : JASN·2026
Same author

Divergent roles of skeletal muscle 11β-HSD1 in age-related and chronic kidney disease-associated sarcopenia.

European journal of endocrinology·2026
Same author

Retraction Note: Delayed administration of pyroglutamate helix B surface peptide (pHBSP), a novel nonerythropoietic analog of erythropoietin, attenuates acute kidney injury.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Cerebrovascular damage caused by the gut microbe/host co-metabolite <i>p</i>-cresol sulfate is prevented by blockade of the EGF receptor.

Gut microbes·2024
Same author

Frequency of dysnatremia in patients admitted with COVID-19 infection and its prognostic implication.

The Journal of international medical research·2023
Same author

Evidence of a causal and modifiable relationship between kidney function and circulating trimethylamine N-oxide.

Nature communications·2023
Same journal

The CD44 protein family: roles in embryogenesis and tumor progression.

Frontiers in bioscience : a journal and virtual library·2017
Same journal

Four varieties of voltage-gated proton channels.

Frontiers in bioscience : a journal and virtual library·2017
Same journal

Lurie's tubercle-count method to test TB vaccine efficacy in rabbits.

Frontiers in bioscience : a journal and virtual library·2017
Same journal

Optical spectroscopy of breast biopsies and human breast cancer xenografts in nude mice.

Frontiers in bioscience : a journal and virtual library·2017
Same journal

The colostrum-deprived, artificially-reared, neonatal pig as a model animal for studying rotavirus gastroenteritis.

Frontiers in bioscience : a journal and virtual library·2017
Same journal

Action of polypeptide growth factors in colon cancer; development of new therapeutic approaches.

Frontiers in bioscience : a journal and virtual library·2017
See all related articles

Endogenous ouabain, despite initial doubts, is now known to trigger significant cellular signaling events at nanomolar concentrations. These findings suggest a crucial physiological role for this compound in human health.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Endogenous ouabain (EO) was first isolated in 1991, but its existence in humans was doubted due to its structure, synthesis, and origin.
  • The physiological relevance of EO was questioned due to its low circulating concentrations (nanomolar range).

Purpose of the Study:

  • To analyze the effects of low-dose, endogenous ouabain-induced signals on cellular processes.
  • To explore the potential physiological roles of endogenous ouabain in humans.

Main Methods:

  • Review and analysis of existing studies on endogenous ouabain.
  • Examination of signal transduction pathways affected by nanomolar ouabain concentrations in cell cultures.

Main Results:

Related Experiment Videos

  • Nanomolar concentrations of ouabain induce significant signal transduction events in various cell types.
  • Observed effects include modulation of cell proliferation, calcium mobilization, cytotoxicity, apoptosis, and mitogen-activated protein kinase (MAPK) activation.

    • Conclusions:

    • Despite initial skepticism, endogenous ouabain plays a significant role in cellular signaling.
    • Low-dose ouabain-induced events support a putative physiological function for endogenous ouabain in humans.