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Updated: Aug 17, 2026

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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
Published on: March 13, 2016
Applications of two-photon microscopy in the neurosciences
Hans G Cruz1, Christian Lüscher
1Department of Basic Neurosciences, University of Geneva, Geneva, Switzerland.
Frontiers in Bioscience : a Journal and Virtual Library
|June 23, 2005
Abstract:
Thanks to deep tissue penetration of infrared light (IR), two-photon laser scanning microscopy (2PLSM) has become an important tool in modern neuroscience. Examples range from the high-resolution visualization of dendritic spines in single neurons to the monitoring of intracellular Ca++ transients in small neural networks. In the present review we give an overview of the current achievements and discuss emerging applications of the technique.

