Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genome size and endonuclear DNA replication in spiders.

Ellen M Rasch1, Barbara A Connelly

  • 1Department of Anatomy & Cell Biology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614-0582, USA. emrash@worldnet.att.net

Journal of Morphology
|June 23, 2005
PubMed
Summary

Arachnid tissue differentiation involves genome amplification, with some spider species exhibiting polysomatic nuclei. This endonuclear DNA replication offers a model for studying developmental variations in DNA content.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ATM deficiency differentially affects expression of proteins related to fatty acid oxidation and oxidative stress in a sex-specific manner in response to Western-type diet prior to and following myocardial infarction.

Life sciences·2024
Same author

Deficiency of ataxia-telangiectasia mutated kinase attenuates Western-type diet-induced cardiac dysfunction in female mice.

Physiological reports·2022
Same author

Deficiency of ataxia-telangiectasia mutated kinase modulates functional and biochemical parameters of the heart in response to Western-type diet.

American journal of physiology. Heart and circulatory physiology·2021
Same author

Comparative study of dentition among species of Poecilia (Pisces).

Journal of morphology·2018
Same author

CLONAL DIVERSITY AND EVOLUTIONARY DYNAMICS IN A DIPLOID-TRIPLOID BREEDING COMPLEX OF UNISEXUAL FISHES (POECILIA).

Evolution; international journal of organic evolution·2017
Same author

EVOLUTIONARY GENETICS OF A GYNOGENETIC FISH, POECILIA FORMOSA, THE AMAZON MOLLY.

Evolution; international journal of organic evolution·2017

Area of Science:

  • Zoology
  • Developmental Biology
  • Genetics

Background:

  • Genome sizes (C-values) are documented for 115 arachnid species.
  • The extent of genome amplification during arachnid tissue differentiation is largely unknown.
  • Arachnids are a diverse and abundant class of invertebrates.

Purpose of the Study:

  • To investigate genome amplification (endonuclear DNA replication or polyploidization) during arachnid tissue differentiation.
  • To determine if polysomatic nuclei arise during development in arachnids.
  • To explore patterns of tissue-specific variation in DNA endoreduplication.

Main Methods:

  • Collected hemolymph and tissue samples from wild-caught arachnids.
  • Prepared air-dried smears and stained them using the Feulgen reaction for DNA.

Related Experiment Videos

  • Assayed DNA content using scanning and image analysis densitometry.

    • Main Results:

    • Giant nuclei with 50-100 pg DNA/nucleus were observed in midgut diverticula and Malpighian tubules of Argiope and Lycosa spiders.
    • These giant nuclei indicate at least four cycles of endonuclear DNA replication compared to hemocytes or sperm.
    • Elevated DNA levels were found less frequently in other species, with no correlation to somatic cell genome sizes.

    Conclusions:

    • Polysomatic nuclei with varying DNA classes regularly emerge during tissue differentiation in some spider species.
    • These findings suggest a potential model system for studying tissue-specific DNA endoreduplication during development.
    • Further research is needed to understand the patterns and implications of this phenomenon in arachnids.