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[Effect of GPI-PLD on adhesion function of bone marrow mononuclear cell from patients with myeloid leukemia and its
Guang-Fen Xiao1, Fang-Ping Chen, Bin Fu
1Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China.
Abstract:
To explore the effect of glycosyl-phosphatidyl inositol-specific phospholipase D (GPI-PLD) on the adhesion function of bone marrow mononuclear cell from patients with myeloid leukemia and analyze its mechanism, the activity of GPI-PLD in bone marrow mononuclear cell from the patients were measured by using GPI-anchored placental alkaline phosphatase (PLAP) as substrate and Triton-X114 partitioning; the adhesion rate and CD24 expression of these cells were measured by MTT and immunohistochemical method respectively, when these cells were or were not treated by 1 mmol/L 1,10-phenanthroline for 5 hours. The results showed that the GPI-PLD activity of bone marrow mononuclear cells from the patients was significantly inhibited after being treated by 1 mmol/L 1, 10-phenanthroline for 5 hours [(42.08 +/- 7.21)% vs (5.4 +/- 2.96)%], while the adhesion rate and the expression of CD24 of these cells were increased [(49.78 +/- 26.73)% vs (61.19 +/- 29.14)%, (16.02 +/- 9.68)% vs (18.5 +/- 11.14)%, respectively)]. It is concluded that depression of GPI-PLD activity can increase the adhesion rate of bone marrow mononuclear cells from the patients while the CD24 expression is enhanced.
Insights
In myeloid leukemia, inhibiting glycosyl-phosphatidyl inositol-specific phospholipase D (GPI-PLD) activity increases bone marrow mononuclear cell adhesion. This suggests GPI-PLD plays a role in myeloid leukemia cell function.
Area of Science:
- Biochemistry
- Hematology
- Cell Biology
Background:
- Myeloid leukemia is characterized by abnormal bone marrow cell function.
- Glycosyl-phosphatidyl inositol-specific phospholipase D (GPI-PLD) is an enzyme implicated in various cellular processes.
- The role of GPI-PLD in the adhesion of myeloid leukemia cells requires further elucidation.
Purpose of the Study:
- To investigate the impact of GPI-PLD activity on the adhesion of bone marrow mononuclear cells from myeloid leukemia patients.
- To analyze the underlying mechanism connecting GPI-PLD activity to cell adhesion and CD24 expression.
Main Methods:
- Measurement of GPI-PLD activity using a GPI-anchored placental alkaline phosphatase (PLAP) substrate and Triton-X114 partitioning.
- Quantification of cell adhesion rates via MTT assay.
- Assessment of CD24 expression using immunohistochemical methods.
- Treatment of cells with 1 mmol/L 1,10-phenanthroline to inhibit GPI-PLD activity.
Main Results:
- Significant inhibition of GPI-PLD activity was observed in treated bone marrow mononuclear cells (5.4 +/- 2.96%) compared to controls (42.08 +/- 7.21%).
- A notable increase in cell adhesion rate was recorded in treated cells (61.19 +/- 29.14%) versus untreated cells (49.78 +/- 26.73%).
- CD24 expression showed a slight increase in treated cells (18.5 +/- 11.14%) compared to controls (16.02 +/- 9.68%).
Conclusions:
- Reduced GPI-PLD activity is associated with enhanced bone marrow mononuclear cell adhesion in myeloid leukemia.
- The findings suggest that modulating GPI-PLD activity could influence myeloid leukemia cell behavior and adhesion properties.
