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Updated: Aug 17, 2026
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
[Analysis of sequence-tagged site in bcr and abl genes by DNA pooling and dHPLC]
Hong Tian1, Dao-Ming Liu, Bing Xu
1Department of Hematology, Fuzhou General Hospital of Nanjing Military Area, Fuzhou 350025, China. tianh710@hotmail.com
Abstract:
To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people.
