Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Organized tooth-specific cellular differentiation stimulated by BMP4.

A Ohazama1, A Tucker, P T Sharpe

  • 1Department of Craniofacial Development, Dental Institute, Kings College London, Floor 28 Guy's Hospital, London Bridge, London SE1 9RT, UK.

Journal of Dental Research
|June 24, 2005
PubMed
Summary

Bone morphogenetic protein 4 (BMP4) can stimulate tooth-specific cell differentiation. This study shows BMP4 can induce dental cell development from non-dental tissues, offering insights into tooth formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Outcomes comparing synthetic and autologous grafts for the reconstruction of the medial patellofemoral ligament. Results of the Syn-VAR prospective randomised trial.

The Knee·2026
Same author

Distinct Involvement of X-Inactivation in Organogenesis.

Journal of dental research·2026
Same author

Wnt/β-catenin Promotes Cementum Apposition in Periodontal Regeneration.

Journal of dental research·2024
Same author

Computational Methods for Image Analysis in Craniofacial Development and Disease.

Journal of dental research·2024
Same author

Redefining Mucosal Inflammation with Spatial Genomics.

Journal of dental research·2024
Same author

Loss of Autophagy Disrupts Stemness of Ameloblast-Lineage Cells in Aging.

Journal of dental research·2023

Area of Science:

  • Developmental biology
  • Molecular signaling in embryogenesis
  • Craniofacial development

Background:

  • Mammalian tooth development originates from reciprocal epithelial-mesenchymal interactions within the first pharyngeal arch.
  • The second pharyngeal arch mesenchyme, typically not involved in tooth formation, can be induced to form teeth when exposed to appropriate signals.
  • Specific signaling molecules, such as BMP4, are expressed by the first pharyngeal arch oral epithelium and are potential inducers of odontogenesis.

Purpose of the Study:

  • To investigate the role of Bone Morphogenetic Protein 4 (BMP4) in inducing tooth development in non-dental embryonic tissues.
  • To determine if BMP4 can stimulate the differentiation of dental-specific cell types from second pharyngeal arch mesenchyme.

Main Methods:

  • Cultured explants of second pharyngeal arch mesenchyme were treated with BMP4.

Related Experiment Videos

  • Analysis of cellular differentiation using marker genes specific for odontoblasts and ameloblasts.

    • Main Results:

    • BMP4 addition to second pharyngeal arch explants induced the formation of organized cellular structures.
    • These structures contained cells expressing marker genes characteristic of odontoblasts and ameloblasts, key dental cell types.
    • While complete tooth formation was not observed, BMP4 demonstrated the capacity to stimulate organized differentiation of dental-specific cells.

    Conclusions:

    • BMP4 is a potent signaling molecule capable of inducing differentiation of dental-specific epithelial and mesenchymal cells from non-dental origins.
    • These findings highlight BMP4's crucial role in initiating the cellular processes underlying tooth development and suggest potential therapeutic applications.