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Synthesis of hydroquinone-alpha-glucoside by alpha-glucosidase from baker's yeast
Radivoje M Prodanović1, Nenad B Milosavić, Dusan Sladić
1Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12, 11000, Belgrade, Serbia and Montenegro. rprodano@chem.bg.ac.yu
Abstract:
Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation reaction were at 30 degrees C for 20 h with 50 mM: hydroquinone and 1.5 M: maltose in 100 mM: sodium citrate/phosphate buffer at pH 5.5. The glucoside was obtained at 0.6 mg/ml with a 4.6% molar yield with respect to hydroquinone.
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