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Dextran dextrinase and dextran of Gluconobacter oxydans
Myriam Naessens1, An Cerdobbel, Wim Soetaert
1Laboratory of Industrial Microbiology and Biocatalysis, Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Ghent, Belgium.
Journal of Industrial Microbiology & Biotechnology
|June 24, 2005
Summary
Gluconobacter oxydans produces dextran dextrinase (DDase), an enzyme that converts maltodextrins into dextrans. Optimized fermentation enhances DDase and dextran production for various applications.
Area of Science:
- Biochemistry
- Enzymology
- Microbial Biotechnology
Background:
- Dextran dextrinase (DDase) from Gluconobacter oxydans converts maltodextrins into dextran.
- Research on DDase, particularly from G. oxydans ATCC 11894, has focused on purification, characterization, and kinetics.
- Understanding the physiological link between intracellular and extracellular DDase is crucial.
Purpose of the Study:
- To provide an overview of the current scientific status of G. oxydans DDase.
- To explore the application potential of DDase and its products, (oligo)dextrins.
- To detail optimized fermentation processes for DDase and dextran production.
Main Methods:
- Optimized fermentation processes for enzyme and product yield.
- Specific assays for measuring DDase activity.
- Characterization of enzyme properties, substrate specificity, and mode of action.
Main Results:
- Two forms of DDase (intracellular and extracellular) are produced by G. oxydans, influenced by fermentation conditions.
- Optimized processes enhance DDase and dextran production.
- DDase functions as a transglucosidase, catalyzing glucosyl transfer to form alpha1,6 linkages.
Conclusions:
- G. oxydans DDase is a versatile biocatalyst with significant application potential.
- Optimized fermentation and enzyme characterization are key to maximizing DDase and dextran production.
- Further research can explore novel applications of DDase-derived products.