Complexities in tetracycline analysis-chemistry, matrix extraction, cleanup, and liquid chromatography
Collin R Anderson1, Heidi S Rupp, Wen-Hsin Wu
1U.S. Food and Drug Administration, Seafood Products Research Center/Pacific Regional Laboratory Northwest, 22201 23rd Drive SE, Bothell, WA 98021, USA. collin.anderson@fda.gov
Abstract:
The extraction and cleanup of commonly used tetracyclines (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) from sample matrix, and their subsequent determination via liquid chromatography can be problematic. Many manuscripts report on various challenges encountered when developing a method for tetracycline antibiotics determination. These complexities often result in less than perfect recoveries or chromatograms and are based on the underlying chemistry associated with tetracyclines. This review compiles, compares, and discusses the results and observations found in published methods, while focusing on chemical principles in order to increase the practicing chemist's understanding of TCs to aid him/her in developing useful analyses.
More Related Videos
Related Concept Videos
Ion-Exchange Chromatography
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
Principles Of Column Chromatography
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:


