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High-performance liquid chromatographic stationary phases based on poly(dimethylsiloxane) immobilized on silica
Edivan Tonhi1, Kenneth E Collins, Carol H Collins
1Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971, Campinas, SP, Brazil.
Abstract:
This work describes the preparation and characterization of six stationary phases for high-performance liquid chromatography (HPLC) obtained by deposition of poly(dimethylsiloxane) (PDMS) in HPLC silica particles, followed by immobilization using different processes (thermal treatments, thermal treatment + microwave irradiation, self-immobilization + gamma irradiation and self-immobilization + microwave irradiation). The chromatographic parameters of all the phases were evaluated with a mixture of test compounds having varied natures (acid, basic and neutral). The stability of one of these phases was evaluated in both a neutral mobile phase and a higher pH mobile phase used at an elevated temperature, with promising results.
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