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Updated: Aug 17, 2026

Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
Neoflavonoids and related constituents from Nepalese propolis and their nitric oxide production inhibitory activity
Suresh Awale1, Suraj Prakash Shrestha, Yasuhiro Tezuka
1Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.
Abstract:
A methanolic extract of Nepalese propolis yielded 10 new open-chain neoflavonoids (1-10), a new chalcone (11), and eight previously reported compounds (12-19). Their structures were determined on the basis of spectroscopic data and chemical conversion. The isolated compounds other than 5, 8, and 16 showed dose-dependent inhibitory activities on nitric oxide production in lipopolysaccharide-activated macrophage-like J774.1 cells and were more active than a positive control, N(G)-monomethyl-L-arginine (IC(50), 27.1 microM). The most potent activities, of 6 and 7 (IC(50), 0.5 microM), were greater than another positive control, caffeic acid phenethyl ester (IC(50), 4.8 microM).
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