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[Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma]
Li-jie Song1, Sheng-long Ye, Kai-feng Wang
1Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Objectives:
To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC).
Methods:
Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using real-time quantitative PCR (RQ-PCR).
Results:
The expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01).
Conclusion:
The expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.
