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Updated: Aug 17, 2026
Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Published on: September 19, 2018
[Differential analysis of tyrosine-phosphorylated proteins in human hepatocellular carcinoma cell lines with
Li-jun Zhang1, Jie-feng Cui, Yin-kun Liu
1Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Objectives:
To compare expressions of tyrosine-phosphorylated proteins in different hepatocellular carcinoma cell lines with different metastasis potential and to screen key molecules associated with HCC metastasis and recurrence.
Methods:
Using two-dimensional electrophoresis, Western blotting and MALDI-TOF-MS/MS, we analyzed tyrosine-phosphorylated protein profiles of Hep3B, MHCC97L and MHCC97H, HCC cell lines with different metastasis potentials.
Results:
10 spots were detected in Hep3B, 19 in MHCC97L and 17 in MHCC97H. Seventeen significantly different phosphotyrosine proteins in gel were identified by MALDI-TOF-MS/MS, including Annexin I.
Conclusion:
The changed expression of tyrosine-phosphorylated proteins is associated with HCC metastasis and recurrence.
