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Related Experiment Videos

Glucocorticoids remodel nuclear envelope structure and permeability.

Victor Shahin1, Yvonne Ludwig, Claudia Schafer

  • 1Institute of Physiology II, University of Münster, Robert-Koch Str. 27b, 48149 Münster, Germany. shahin@uni-muenster.de

Journal of Cell Science
|June 25, 2005
PubMed
Summary

Glucocorticoids rapidly remodel the nuclear envelope (NE) and nuclear pore complexes (NPCs) in Xenopus oocytes. These structural changes transiently increase NE permeability, facilitating glucocorticoid actions.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • The nuclear envelope (NE) regulates transport between the nucleus and cytoplasm.
  • Glucocorticoids are steroid hormones with diverse physiological effects.
  • The dynamic structural changes of the NE in response to hormones are not fully understood.

Purpose of the Study:

  • To investigate the effects of glucocorticoids on the structure and permeability of the nuclear envelope.
  • To elucidate the role of nuclear pore complexes (NPCs) in hormone-induced NE remodeling.

Main Methods:

  • Xenopus laevis oocytes expressing glucocorticoid receptors (GR) were treated with triamcinolone acetonide (TA).
  • Nuclear envelope permeability and structure were assessed using electrical measurements, fluorescence microscopy, and nano-imaging techniques.

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  • Changes were analyzed at 5 and 60 minutes post-treatment.

    • Main Results:

    • Within 5 minutes, TA induced NPC dilation, altered NPC distribution, and increased NE permeability to ions and dextran.
    • NPCs formed clusters, and the hydrophobicity of NPC channels and NE surface increased.
    • These NE alterations were transient, returning to baseline within 60 minutes.

    Conclusions:

    • The nuclear envelope exhibits significant plasticity and is sensitive to hydrophobic molecules like glucocorticoids.
    • Glucocorticoid-induced NE remodeling, including NPC dynamics, is a crucial early step in mediating hormone actions.
    • This study reveals a rapid, dynamic mechanism for nuclear transport regulation by hormones.