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15:31
MISSION esiRNA for RNAi Screening in Mammalian Cells
Published on: May 12, 2010
Fast screening target sites for RNA interference using a cell-free system
Huang Mei1, Jia Fang-Jun, Li Chong-Yong
1Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, China.
Biotechnology Letters
|June 25, 2005
Abstract:
A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but which are more time-consuming than this present method.
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01:23RNA Interference
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