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Updated: Aug 17, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
Published on: June 11, 2015
Recombinant extracellular matrix-like proteins with repetitive elastin or collagen-like functional motifs
Hiroyuki Kurihara1, Tetsuaki Morita, Masashige Shinkai
1School of Engineering, The University of Tokyo, Bunkyo-ku, 113-8656 Tokyo, Japan.
Abstract:
Using overlap elongation PCR, we created repetitive DNA libraries encoding the elastin VPGVG and collagen-like GERGDRGDP sequences. From these libraries we isolated two repetitive DNA sequences, Col-5 encoding [(GERGDRGDP)(5)GER], and Ela-16 encoding [(VPGVG)(16)VPG]. Both proteins were expressed as thioredoxin fusion proteins. The resulting recombinant extracellular matrix-like proteins had the expected properties (cell adhesive ability and thermally responsive structural change) of the functional motif sequence unit used.
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