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Related Experiment Videos

Single genes from Agrobacterium rhizogenes influence plant development.

T Schmülling1, J Schell, A Spena

  • 1MPI für Züchtungsforschung, 5000 Köln, 30, FRG.

The EMBO Journal
|September 1, 1988
PubMed
Summary

Agrobacterium rhizogenes rol genes induce hairy-root syndrome in tobacco. Individual rol genes cause distinct developmental issues, while combined action of rol A, B, and C genes is crucial for efficient root growth.

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Area of Science:

  • Plant molecular biology
  • Genetics
  • Agrobacterium research

Background:

  • Agrobacterium rhizogenes Ri-plasmids contain rol genes (A, B, C) that influence plant development.
  • Hairy-root syndrome is a pathological state induced by the combined expression of these rol genes in transgenic plants.

Purpose of the Study:

  • To investigate the individual and combined effects of rol A, B, and C genes on plant development.
  • To explore how altering gene expression, using the cauliflower mosaic virus 35S promoter, impacts developmental patterns.

Main Methods:

  • Transgenic tobacco plants were created expressing rol A, B, and C genes individually and in combination.
  • Gene expression was modified by replacing native promoters with the cauliflower mosaic virus 35S promoter for rol B and C genes.

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Main Results:

  • Individual rol genes elicit distinct developmental abnormalities in tobacco.
  • Modified expression of rol B and C genes using the 35S promoter results in novel developmental patterns.
  • While each rol gene can promote root formation, combined activity of multiple rol genes is necessary for efficient root initiation and growth.

Conclusions:

  • Rol gene products have distinct targets or qualitatively different effects on common targets involved in plant development control.
  • The combined action of Agrobacterium rhizogenes-derived TL-DNA genes is essential for optimal root development in tobacco.