Combined effect of chloroquine and insulin administration on some biochemical parameters in rats placed on high fat...

Effect of combined administration of Insulin and Chloroquine on fasting blood glucose, total protein, creatinine and uric acid concentration were investigated in rats placed on diets high in fat and calcium (SP diet). Thirty-six (36) rats (grouped into six) were placed on different treatment: Grams A and B were fed with NP (normal diet) and SP diet respectively; Group C was placed on NP diet and injected intramuscularly with 100 microg insulin per day; Group D was placed on SP diet and also injected with 100 microg insulin per day; Group E was placed on SP diet and injected with both insulin (100 microg/day) and chloroquine (20mg/Kg thrice weekly); Group F was placed on SP diet and injected with chloroquine (20mg/Kg) thrice weekly. After 15 weeks of treatment, a significantly reduced concentration of glucose was observed in groups injected with insulin and those injected with insulin and chloroquine together (compared with the control groups, A and B). The serum total protein and uric acid level were however not significantly different in all the rats. Serum creatinine was also observed to be significantly lowered in the rats treated with insulin. The results of this study thus suggest that insulin and chloroquine administration may result in reduced blood glucose level (hypoglycemia). It also suggests that insulin and chloroquine administration may further effect an improved kidney function.