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Updated: Aug 17, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
Published on: November 26, 2013
Creating new specific ligand-receptor pairs for transgene regulation
Karuppiah Chockalingam1, Huimin Zhao
1Department of Chemical Engineering and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801, USA.
Abstract:
The creation of specifically matched ligand-receptor pairs that are orthogonal to naturally present interacting pairs is essential for the development of small molecule-regulated gene expression systems for biotechnological applications. However, for many years this task has represented a significant challenge for synthetic chemists and protein engineers. Recently, Doyle and colleagues demonstrated that highly specific ligand-receptor pairs can be engineered in a rapid fashion by creating large libraries of protein variants and applying a selection scheme to identify variants with improved activation by the target synthetic ligand.
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