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Related Experiment Videos

Sludge based Bacillus thuringiensis biopesticides: viscosity impacts.

S K Brar1, M Verma, R D Tyagi

  • 1INRS-Eau, Terre et Environnement (INRS-ETE), 2800 Rue Einstein, CP 7500, Sainte-Foy, Quebec, Canada G1V 4C7.

Water Research
|June 28, 2005
PubMed
Summary

Pre-treating Bacillus thuringiensis (Bt) sludge with heat and alkaline hydrolysis reduces viscosity and increases biopesticide effectiveness. These findings optimize Bt sludge fermentation and formulation for biopesticide production.

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The Poly(3-Hydroxybutyrate) Production From the Mixed Culture With the Leachate Obtained From Wastewater Sludge Pretreated by the Electrochemical Process.

Biotechnology and applied biochemistry·2025

Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Agricultural Science

Background:

  • Bacillus thuringiensis (Bt) is crucial for biopesticide production.
  • Optimizing sludge properties is key for efficient biopesticide formulation.
  • Rheological characteristics of sludge impact bioprocess outcomes.

Purpose of the Study:

  • To investigate the impact of pre-treatment methods on the viscosity of Bt fermented sludges.
  • To correlate rheological parameters with solids concentration and entomotoxicity (Tx).
  • To evaluate the influence of pre-treatments on nutrient availability and oxygen transfer for enhanced biopesticide production.

Main Methods:

  • Viscosity measurements of raw and pre-treated Bt sludges at varying solids concentrations (10-40 g/L).

Related Experiment Videos

  • Analysis of total and dissolved solids, soluble chemical oxygen demand (COD).
  • Determination of entomotoxicity (Tx) and reproducibility in shake flasks and fermenters.

    • Main Results:

    • Hydrolysed fermented sludges followed exponential and power laws, unlike raw sludge.
    • Pre-treatments increased dissolved solids and soluble COD, altering viscosity.
    • Hydrolysed fermented sludge exhibited higher Tx due to improved nutrient availability and lower viscosity, enhancing oxygen transfer.

    Conclusions:

    • Pre-treatment significantly modifies Bt sludge rheology and enhances biopesticide efficacy.
    • Reduced viscosity in pre-treated sludge facilitates better oxygen transfer, crucial for Bt growth and toxin production.
    • Findings provide a basis for optimizing fermentation, harvesting, and formulation techniques for Bt biopesticides.