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Related Experiment Video

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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
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Published on: February 22, 2016

Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling.

C Machka1, M Kersten, M Zobawa

  • 1Institute of Experimental Genetics, GSF-National Research Center, Ingolstädter Landstr.1, 85764 Neuherberg, Germany.

Gene Expression Patterns : GEP
|June 28, 2005
PubMed
Summary

The Notch signaling pathway regulates embryonic development. This study identified novel genes and proteins affected by Dll1 mutations, expanding our understanding of Notch signaling in embryogenesis.

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Last Updated: Jul 16, 2026

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The Notch signaling pathway plays crucial roles in mammalian embryogenesis, particularly in patterning and differentiation.
  • It is essential for the development of paraxial mesoderm and the neural tube.

Purpose of the Study:

  • To identify genes and proteins differentially regulated in Dll1 mutant embryos using comprehensive expression profiling.
  • To uncover novel targets and downstream effects of Notch signaling during embryonic development.

Main Methods:

  • DNA-chip expression profiling to identify regulated transcripts.
  • 2D-gel electrophoresis and peptide mass fingerprinting to identify differentially expressed proteins.
  • Whole-mount in situ hybridization and immunoblots for validation.

Main Results:

  • Identified 47 regulated transcripts and 40 differentially expressed proteins in Dll1 mutant embryos.
  • Many identified genes were not previously linked to Notch signaling.
  • Expression patterns of identified genes were altered in key embryonic tissues, including the presomitic mesoderm, somites, and neural tube.
  • Six proteasome subunits were found to be down-regulated in Dll1 mutant embryos.

Conclusions:

  • This study provides a substantial resource of novel genes and proteins regulated by Notch signaling.
  • The findings offer new avenues for investigating the complex roles of Notch in embryonic development.
  • The identification of proteasome subunits suggests a link between Notch signaling and protein degradation pathways.