Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of detection methods for ruminant interleukin (IL)-4.

J C Hope1, L S Kwong, M Thom

  • 1Institute for Animal Health, Compton, Near Newbury, RG20 7NN, UK. Jayne.Hope@BBSRC.ac.uk

Journal of Immunological Methods
|June 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of individual differences and attitude in willingness to participate in TMS studies.

Behavior research methods·2025
Same author

Phenotype of bovine mononuclear phagocytes- An update.

Veterinary immunology and immunopathology·2024
Same author

Informal sale of antibiotics in Guatemalan convenience stores before and after implementation of federal antibiotic dispensing legislation.

BMC pharmacology & toxicology·2024
Same author

Histological and immunohistochemical features suggesting aetiological differences in lymph node and (muco)cutaneous feline tuberculosis lesions.

The Journal of small animal practice·2021
Same author

Ferroelasticity, anelasticity and magnetoelastic relaxation in Co-doped iron pnictide: Ba(Fe<sub>0.957</sub>Co<sub>0.043</sub>)<sub>2</sub>As<sub>2</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2019
Same author

Cytokine and Chemokine Concentrations as Biomarkers of Feline Mycobacteriosis.

Scientific reports·2018
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Researchers developed a new assay to detect bovine interleukin-4 (IL-4) in animals. This method enables the study of IL-4

Area of Science:

  • Veterinary Immunology
  • Molecular Biology
  • Immunotechnology

Background:

  • Interleukin-4 (IL-4) is a critical cytokine in immune responses, particularly in parasitic and allergic diseases.
  • Detecting ruminant IL-4 is essential for understanding animal health and disease pathogenesis.
  • Existing methods for IL-4 detection may not be suitable for all ruminant applications.

Purpose of the Study:

  • To develop and validate a sensitive assay for detecting recombinant and native bovine IL-4.
  • To establish a method for quantifying IL-4 production in cellular immune responses.
  • To enable further research into the role of IL-4 in ruminant immunology and disease.

Main Methods:

  • Generation of monoclonal antibodies (mAb) against recombinant bovine IL-4 (rbo IL-4).

Related Experiment Videos

  • Development of an ELISA using a specific pair of mAb (CC313 and CC314) for IL-4 detection.
  • Application of luminometric detection, ELISPOT, and intracellular cytokine staining (flow cytometry) for IL-4 analysis.

    • Main Results:

    • A pair of mAb (CC313 and CC314) successfully detected both recombinant and native bovine IL-4 via ELISA.
    • Native bovine IL-4 was detected in peripheral blood mononuclear cell (PBMC) supernatants after mitogen stimulation.
    • High IL-4 secretion was confirmed in Fasciola hepatica-specific Th2 clones, but not Babesia bovis-specific Th1 clones.
    • The assay also detected recombinant ovine IL-4 and enabled IL-4 detection in ELISPOT and intracellular staining.

    Conclusions:

    • A robust method for detecting ruminant IL-4 using ELISA, ELISPOT, and flow cytometry has been established.
    • This assay facilitates the study of IL-4's role in the immunology and pathogenesis of animal diseases.
    • The developed tools will advance research on immune responses in livestock.