Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Progress on gene targeting].

Hong-quan Liu1, Ji-xun Dai, Wen-gong Yu

  • 1Ocean University of Qingdao, College of Marine Life Sciences, Qingdao 266003, China. lhongquan@163.com

Yi Chuan = Hereditas
|June 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and functional characterization of two novel hyaluronate lyases BxHly33 and BiHly33, from the human gut microbiome.

International journal of biological macromolecules·2025
Same author

Effect and mechanism of oritavancin on hIAPP amyloid formation.

Journal of materials chemistry. B·2025
Same author

Chitosan oligosaccharides alleviate macrophage pyroptosis and protect sepsis mice via activating the Nrf2/GPX4 pathway.

International journal of biological macromolecules·2024
Same author

Clinical efficacy of Baijin pills in the treatment of generalized tonic-clonic seizure epilepsy with cognitive impairment.

World journal of psychiatry·2024
Same author

Endotoxin accelerates insulin amyloid formation and inactivates insulin signal transduction.

Life sciences·2023
Same author

Corrigendum to "Transcriptome analysis reveals possible antitumor mechanism of Chlorella exopolysaccharide" [Gene 779 (2021) 145494].

Gene·2021
Same journal

Construction and implementation of an ICF-based integrated teaching model for genetic disease severity assessment.

Yi chuan = Hereditas·2026
Same journal

Identification and prenatal genetic testing of pathogenic variants in a case of myoclonus-dystonia syndrome.

Yi chuan = Hereditas·2026
Same journal

A novel strategy to enhance precise targeting of the RNA base editor mxABE.

Yi chuan = Hereditas·2026
Same journal

Functional study of the soybean rapid alkalinization factor <i>GmRALF34s</i> in response to saline-alkali stress.

Yi chuan = Hereditas·2026
Same journal

Role of <i>broad</i> in intestinal stem cells of adult <i>Drosophila</i>.

Yi chuan = Hereditas·2026
Same journal

The p53 R267W mutation intervenes p21-mediated cell cycle arrest and promotes proliferation and migration of lung cancer cells.

Yi chuan = Hereditas·2026
See all related articles

Gene targeting, a key molecular biology technique, precisely modifies cell genomes. This review explores its principles, applications, and methods to overcome low efficiency challenges in gene editing.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Context:

  • Gene targeting utilizes homologous recombination to introduce exogenous DNA at specific genomic sites.
  • This technology is crucial for advancing research in developmental biology, molecular genetics, immunology, and medicine.

Purpose:

  • To review the fundamental principles and methodologies of gene targeting.
  • To discuss current challenges, particularly the low efficiency of the process.
  • To explore potential strategies for enhancing gene targeting efficiency.

Summary:

  • Gene targeting enables precise modification of cellular hereditary characteristics.
  • Applications include gene structure/function studies, gene expression analysis, and gene therapy development.
  • Low efficiency remains a significant hurdle in widespread gene targeting applications.

Related Experiment Videos

Impact:

  • Facilitates deeper understanding of gene function and regulation.
  • Opens avenues for novel therapeutic strategies, including gene therapy.
  • Aims to improve the efficiency and applicability of gene editing technologies.