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Updated: Aug 17, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Published on: February 5, 2019
[A novel approach to study pathogenesis of pathogens in vivo--signature-tagged mutagenesis]
Xiao Yao1, Liu-yu Huang, Bo-lun Yang
1Beijing Institute of Biotechnoloy, Beijing 100071, China. yaoxiao50@hotmail.com
Abstract:
Signature-tagged mutagenesis (STM) is a novel approach to study pathogenesis of pathogens and to screen virulence genes with high throughput in vivo,which is based on whole genome of pathogen in question. In recent years, more than ten species of microbial pathogens have been screened with this technology. There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens. This article reviews the principle, advantages and current limitations,the requirements, modifications of STM, and to date virulence genes identified by this technology.
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