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Updated: Aug 17, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
Published on: November 3, 2011
Scooby-domain: prediction of globular domains in protein sequence
Richard A George1, Kuang Lin, Jaap Heringa
1Inpharmatica Ltd, 60 Charlotte Street, London W1T 2NU, UK.
Abstract:
Scooby-domain (sequence hydrophobicity predicts domains) is a fast and simple method to identify globular domains in protein sequence, based on the observed lengths and hydrophobicities of domains from proteins with known tertiary structure. The prediction method successfully identifies sequence regions that will form a globular structure and those that are likely to be unstructured. The method does not rely on homology searches and, therefore, can identify previously unknown domains for structural elucidation. Scooby-domain is available as a Java applet at http://ibivu.cs.vu.nl/programs/scoobywww. It may be used to visualize local properties within a protein sequence, such as average hydrophobicity, secondary structure propensity and domain boundaries, as well as being a method for fast domain assignment of large sequence sets.
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