Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The multilocus sequence typing network: mlst.net.

David M Aanensen1, Brian G Spratt

  • 1Department of Infectious Disease Epidemiology, Imperial College London, St Mary's Hospital, London W2 1PG, UK. d.aanensen@imperial.ac.uk

Nucleic Acids Research
|June 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Type 3 fimbrial regulation underpins anti-MrkA immunotherapeutic efficacy in experimental Klebsiella pneumoniae infection.

The Journal of infectious diseases·2026
Same author

Life Identification Numbers: A strain nomenclature approach to aid epidemiological surveillance of bacterial pathogens.

PLoS biology·2026
Same author

Emergence of carbapenemase-producing Escherichia coli in acute care hospitals in 32 European countries (the CCRE survey): a prospective, multicentre, cross-sectional, epidemiological, microbiological, and genomic surveillance study.

The Lancet. Microbe·2026
Same author

Persistent spread of carbapenemase-producing Klebsiella pneumoniae in acute care hospitals in 36 European countries (the CCRE survey): a prospective, multicentre, cross-sectional, epidemiological, microbiological, and genomic surveillance study.

The Lancet. Microbe·2026
Same author

Convergence and global molecular epidemiology of Klebsiella pneumoniae plasmids harbouring the iuc3 virulence locus: a population genomic analysis.

The Lancet. Microbe·2026
Same author

Identification of genetic determinants that drive population differentiation and host adaptation in <i>Campylobacter jejuni</i> in California.

Microbial genomics·2025
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Multilocus sequence typing (MLST) provides unambiguous strain characterization for pathogens. This method enables global comparison of microbial populations and evolutionary studies through standardized genetic analysis.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Accurate pathogen strain characterization is vital for understanding epidemiology, population dynamics, and evolution.
  • Multilocus sequence typing (MLST) has emerged as a preferred molecular typing method for bacterial and fungal pathogens.
  • MLST relies on sequencing seven housekeeping gene loci for unambiguous strain identification.

Purpose of the Study:

  • To highlight the importance and utility of MLST for microbial strain characterization.
  • To introduce a network of MLST schemes and associated databases for global strain comparison.
  • To provide tools for analyzing strain relationships, population structure, and evolutionary patterns.

Main Methods:

  • Utilizing sequence data from seven housekeeping gene fragments for strain definition.

Related Experiment Videos

  • Employing internet-based platforms for data submission and comparison between laboratories.
  • Implementing analytical tools such as allelic profile clustering, phylogenetic trees, and eBURST for population analysis.

    • Main Results:

    • MLST offers unambiguous and globally comparable strain characterization through standardized genetic sequencing.
    • A network of MLST websites (http://www.mlst.net) provides access to schemes and databases for numerous organisms.
    • Tools are available for characterizing strains, relating them to existing databases, and visualizing population structures (e.g., clonal complexes via eBURST).

    Conclusions:

    • MLST is an effective and widely adopted method for microbial strain typing and population studies.
    • The MLST network facilitates international collaboration and data sharing for microbial epidemiology and evolution.
    • Advanced analytical methods integrated within MLST schemes enhance the understanding of microbial population structures.