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Updated: Aug 17, 2026
Intradermal Microdialysis: An Approach to Investigating Novel Mechanisms of Microvascular Dysfunction in Humans
Published on: July 21, 2023
Age-dependent change of uric acid level in the dermis using cutaneous microdialysis
Yong Suk Lee1, Jeong Hoon Yang, Jung Chul Choi
1Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
Background:
We had proposed the usefulness of cutaneous microdialysis for the study of antioxidants in the skin.
Objective:
We designed a study analyzing the level of uric acid in the skin, one of the major antioxidants, for an age-dependent change.
Methods:
16 healthy male volunteers were divided into two groups according to age. Eleven subjects were in their 3rd decade, under 30 years of age (young group) and the others were their 8th decade (old group), over 70 years of age. Dialysate samples were analyzed using high-performance liquid chromatography analysis.
Results:
In the young group the mean level of uric acid was 31.9+/-16.1 microg/ml, while in old group it was 13.4+/-5.2 microg/ml.
Conclusion:
This result demonstrated an in vivo state of antioxidant level in the human skin and the age-dependent difference was concordant with other in vitro or ex vivo studies; therefore, cutaneous microdialysis could be used in analysis and monitoring studies including human antioxidants and anti-aging.
