Related Experiment Video
Updated: Aug 17, 2026

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
Published on: June 30, 2022
A single active-site region for a group II intron
Alexandre de Lencastre1, Stephanie Hamill, Anna Marie Pyle
1Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th St., New York, New York 10032, USA.
Abstract:
Despite the biological importance of self-splicing group II introns, little is known about their structural organization. Synthetic incorporation of site-specific photo-cross-linkers within catalytic domains resulted in functional distance constraints that, when combined with known tertiary interactions, provide a three-dimensional view of the active intron architecture. All functionalities important for both steps of splicing are proximal before the first step, suggestive of a single active-site region for group II intron catalysis.
Related Concept Videos
RNA Splicing
RNA Splicing
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Non-LTR Retrotransposons
Cis-regulatory Sequences

