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[Effect of ginsenoside Rg1 on neprilysin expression induced by LPS in SK-N-SH cell line]
Junhua Lü1, Feng Huang, Yueli Liu
1Jinan University, Guangzhou 510632.
Objective:
To investigate the effect of ginsenoside Rg1 on the neprilysin (NeP) expression induced by LPS in SK-N-SH cell line.
Methods:
MTT was used to measure the survival rate of SK-N-SH cultured with ginsenoside Rg1 at different concentrations (5, 10, 20 micromol/L) and LPS (50 mg/L). The expression of NEP was measured by RT-PCR.
Result:
The survival rate of SK-N-SH was obviously inhibited by LPS, the expression of NEP was decreased. On the other hand, the above alteration induced by LPS was reversed by ginsenoside Rg1.
Conclusion:
This study demonstrates that LPS can cause cell damage and the decrease of NEP expression. Ginsenoside Rg1 can protect SK-N-SH cells against the injury induced by LPS, increase NEP expression.