Related Experiment Video
Updated: Aug 17, 2026
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
Published on: June 17, 2015
Recent developments in preclinical toxicological pathology
1Charles River Laboratories, Preclinical Services, Edinburgh, EH33 2NE, UK. john.finch@eur.crl
Abstract:
In the late nineteenth century, microscopists developed a quaint method for examining the fine structure of biological specimens: paraffin embedding and staining with hematoxylin and eosin. This ancient technology is here to stay for the foreseeable future, because it can and does reveal the truth about biological processes. However, the role of pathology is developing with ever greater world wide interaction between pathologists, and better communication and agreeing of international standards. Furthermore, recent techniques including immunohistochemistry, electron microscopy and image analysis complement the traditional tried and tested tools. There is also in toxicologic pathology a willingness to use pathology methods and skills in new contexts, drug discovery in particular. But even in these days of genetic modification, proteomics and high throughput screening, pathologists continue to rely on dyes extracted from a Central American logwood used in Mexico before the Spanish invasion in 1520.
Insights
Hematoxylin and eosin staining, a classic microscopy technique, remains vital for understanding biological processes. Modern pathology integrates this method with advanced tools like immunohistochemistry and electron microscopy for comprehensive analysis.
Area of Science:
- Histopathology
- Toxicologic Pathology
- Biomedical Research
Background:
- The late 19th century saw the development of paraffin embedding and hematoxylin and eosin (H&E) staining for examining biological specimen fine structure.
- This traditional histological technique remains a cornerstone in pathology due to its effectiveness in revealing biological truths.
Purpose of the Study:
- To highlight the enduring relevance of traditional H&E staining in modern pathology.
- To discuss the integration of H&E staining with contemporary techniques in pathology.
- To explore the application of pathology methods in new fields like drug discovery.
Main Methods:
- Paraffin embedding and H&E staining of biological specimens.
- Complementary use of immunohistochemistry, electron microscopy, and image analysis.
- Application of pathology methods in toxicologic pathology and drug discovery.
Main Results:
- H&E staining continues to be a reliable method for analyzing biological processes.
- International collaboration and standardization are enhancing the field of pathology.
- Advanced techniques effectively complement traditional histological methods.
Conclusions:
- Despite advancements, H&E staining remains indispensable in pathology.
- The field of pathology is evolving with increased global interaction and new technological integrations.
- Pathology methods are adaptable to emerging areas such as drug discovery and toxicologic pathology.
Related Concept Videos
01:23Toxicity Testing in Animals
01:28Preclinical Development: Overview