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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
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A simple flow injection spectrophotometric determination of nitrite based on its reaction with thiourea

Mohammad Pourhossein1, Mohammad K Amini, Morteza Talebi

  • 1Chemistry Department, University of Isfahan, Isfahan, 81746-73441, Iran.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|June 30, 2005
PubMed

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The precipitation titration curve demonstrates the change in concentration of one reactant with the volume of titrant added. During the titration of chloride ions with silver nitrate, the precipitation titration curve is divided into three regions: before, at, and after the equivalence point. Before the equivalence point, low redissolution of the sparingly soluble silver chloride precipitate gives a low silver ion concentration. However, in the second region, representing the equivalence point,...
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Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.